INVESTIGADORES
GIL Jose Fernando
artículos
Título:
TcTASV Antigens of Trypanosoma cruzi: Utility for Diagnosis and High Accuracy as Biomarkers of Treatment Efficacy in Pediatric Patients
Autor/es:
FLORIDIA-YAPUR, N; MONJE RUMI, MM; RAGONE, PG; LAUTHIER, J; TOMASINI, N; ALBERTI D´AMATO, AM; DIOSQUE, P; CIMINO, RO; GIL, JF; SANCHEZ, D; NASSER, JR; TEKIEL, V
Revista:
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
Editorial:
AMER SOC TROP MED & HYGIENE
Referencias:
Lugar: Stanford; Año: 2019 vol. 101 p. 1135 - 1138
ISSN:
0002-9637
Resumen:
The discovery and characterization of novel parasite antigens to improve the diagnosis of Trypanosoma cruzi by serological methods and for accurate and rapid follow-up of treatment efficiency are still needed. TcTASV is a T. cruzi-specific multigene family, whose products are expressed on the parasite stages present in the vertebrate host. In a previous work, a mix of antigens from subfamilies TcTASV-A and TcTASV-C (Mix A + C) was sensitive and specific to identify dogs with active infection of high epidemiological relevance. Here, TcTASV-A and TcTASV-C were assayed separately as well as together (Mix A + C) in an ELISA format on human samples. The Mix A + C presented moderate sensitivity (78%) but high diagnostic accuracy with a 100% of specificity, evaluated on healthy, leishmaniasic, and Strongyloides stercoralis infected patients. Moreover, antibody levels of pediatric patients showed-2 years posttreatment-diminished reactivity against the Mix A + C (P < 0.0001), pointing TcTASV antigens as promising tools for treatment follow-up.