Immunoenzimatic evaluation of the recombinant TSSA-II protein of Trypanosoma cruzi in dogs and human sera: A tool for epidemiologic studies

CIMINO, RO; MONJE RUMI, MM; RAGONE, PG; LAUTHIER, J.; ALBERTI D´AMATO, A; LOPEZ QUIROGA, I; GIL, JF; CAJAL, SP; ACOSTA, N; JUAREZ, M; KROLEWIECKI, AJ; ORELLANA, V; ZACCA, R; MARCIPAR, I; DIOSQUE, P; NASSER, JR

PARASITOLOGY

CAMBRIDGE UNIV PRESS

Año: 2011 vol. 26 p. 1 - 8

0031-1820

The rTSSA-II (recombinant Trypomastigote Small Surface) antigen was evaluated by ELISA technique to detect anti-Trypanosoma cruzi antibodies in sera from naturally infected dogs and humans. For this evaluation ELISA-rTSSA-II was standardized and groups were classified according to the results obtained through xenodiagnosis, ELISA and PCR. Sensitivity (Se), Specificity (Sp), Kappa index (IK) and area under curve (AUC) area were determined. The Se was determined by using 14 sera from dogs infected with T. cruzi VI (TcVI) whereas Sp, by using 95 non-chagasic sera by xenodiagnosis, ELISA- Homogenate and PCR. The performance of ELISA-rTSSA-II in dog sera was high (AUC=0.93 and IK=0.91). The Se was 92.85% (one false negative) and Sp was 100%. Two sera from dogs infected with TcI and one with TcIII were negative. For patients infected with T. cruzi, reactivity was 87.8% (36/41), there was only one indeterminate, and Sp was 100%. 54 sera from non-chagasic and 68 sera from patients with cutaneous leishmaniasis did not react with rTSS-II. ELISA-rTSSA-II showed a high performance when studying sera from naturally infected dogs and it also presented 100% Sp.This assay could be an important tool to carry out sero37 epidemiologic surveys on the prevalence of T. cruzi circulant lineage in the region.