ANTIBACTERIAL AND ANTIFUNGICAL PROPERTIES OF ESSENTIAL OILS OF ALOYSIA TRIPHYLLA

BELTRAMINO, M., OLIVA, M., GALLUCCI, N., ZYGADLO, J., DEMO, M

Huerta Grande (Cba).

Otro; I Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

ANTIBACTERIAL AND ANTIFUNGICAL PROPERTIES OF ESSENTIAL OILS OF ALOYSIA TRIPHYLLA Beltramino M, Oliva M, Gallucci N, Zygadlo J, Demo M. UNRC. Fac. Cs. Ex., Fc-Qcas. y Nat. Dpto. Microbiol. Inmunol. e-mail:moliva@exa.unrc.edu.ar Introduction: A.triphylla (cedrón) is an aromatic shrub that wildly grows from the north to the center of Argentine country and is cultivated from Mexico to South América. It is popularly used as digestive, antiinflamatory, analgesic and antipyretic. It is considered in the national and international market because of the  Objetives: To study the antimicrobial activity (AA) of the EO of A.triphylla collected from different places. Materials and Methods: EO were obtained by hydrodestillation methods from vegetables collected in plantations located in Río 1º, La Paz, Las Viñas, Paraguay, Mendoza and San Luis. The AA was evaluated with the disc diffusion technique and the broth microdilution method, using bacterial and yeasts strains. The Minimun Bactericidal and Fungicidal Concentration were determined (MBC/MFC)). Results and Discussion: All the EOs were more active against Gram positive bacteria, while Gram negative ones were inhibited by half of the EOs. The yeasts were inhibited by all the EOs. The oils from Cba (La Paz and Rio 1º) showed the most effective AA. The best Minimun Inhibitory Concentration (MIC) values were obtained with La Paz EO on S.aureus and S.epidermidis (MIC=28,12mg/ml), for B.cereus, M.luteus, Hansenula sp and Rhodotorula sp (MIC=7,03mg/ml). It inhibited all the Gram negative bacteria, with the exception of P.aeruginosa. La Paz EO was bactericidal for S. aureus and fungicidal for C. albicans while the rest of EOs were bacteriostatic and fungistatic.