MINIMUN INHIBITORY CONCENTRATION OF LEVOFLOXACINE ON ENTEROPATHOGENIC STRAINS OBTAINED FROM ANIMALS

FREYTES S, OLIVA M, ERRECALDE C, PRIETO G, MAÑAS F, DEMO M, GARCÍA OVANDO H

Huerta Grande (Cba).

Otro; I Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

MINIMUN INHIBITORY CONCENTRATION OF LEVOFLOXACINE ON ENTEROPATHOGENIC STRAINS OBTAINED FROM ANIMALS Freytes S, Oliva M, Errecalde C, Prieto G, Mañas F, Demo M, García Ovando H. Dpto Microbiol e Inmunol, Fac Cs Ex, Fco-Qca y Nat, UNRC. Río IV, Córdoba, Argentina. sebafrey@hotmail.com Introduction: Levofloxacine (LFX) is a wide spectrum antimicrobial. Its human terapheutical properties and the kinetic in animals justify the study of its activity on pathogenic strains isolated from domestic animals. Objetive: Determination of antimicrobial activity of LFX on strains isolated from domestic animals Materials and methods: The Minimun Inhibitory Concentration (MIC) was performed by the agar dilution method proposed by the NCCLS against E.coli (N=14) and Salmonella sp (N=8) isolated of clinical cases of pecuary stablishments of the south of Córdoba. It used a reference strain of E. coli ATCC 25922. Concentrations ranging from 6,25 to 0,02mg/ml of LFX were incorporated to Mueller-Hinton agar. The bacterial density was 0,5 of McFarland scale and it was inoculated by a Steers-Foltz multiinoculator. Plates were incubated 24 h, at 37ºC.. The strains able to growth at concentrations equal or bigger than 2 mg/ml were considered resistant. Results and discussion: All Salmonella sp. and twelve E. coli strains tested were sensible to LFX while two E.coli strains were resistant. These results show that LFX is a therapeutical alternative to treat animal patologies caused by enterobacteria since MIC values are similar to the reported for human and lower to plasmatic and tissular levels of LFX in animals.