10. Antimicrobial relationship between terpenes and the penicillin resistant Staphylococcus aureus meticillin resistant (MRSA).

GALLUCCI, N.; OLIVA, M.; CACCIABUE, M.; SABINI, L.; ZYGADLO, J.; DEMO, M

Villa Giardino

Jornada; XV Jornadas Científicas de la Sociedad de Biología de Córdoba; 2005

Antimicrobial relationship between terpenes and the penicillin over meticilin resistant Staphylococcus aureus Gallucci, N., Oliva, M.; Cacciabue, M.; Sabini, L.; Zygadlo, J.; Demo, M. Dpto de Microbiol. e Inmunol..UNRC                                                                                       Medicinal plants are able to sinthetise aromatic compounds such as essential oils. These oils are complex mixtures of organic molecules like monoterpenes and sesquiterpenes. The EO as well as terpenes have antimicrobial activity. In the last years several microbial strains have adquired resistance against antibiotic, so there is a growing interest to find new drugs against bacterias such as meticillin resistant Staphylococcus aureus (MRSA). Aims: To evaluate terpenes antimicrobial activity and to determine the synergistic or antagonic relationship between these and penicillin. Materials and Methods: 8 terpenes were tested against S.aureus ATCC 25212 Meticillin sensitive (MSSA) and Meticillin resistant S. aureus ATCC 25923. Minimun Inhibitory Concentration (CIM) and Minimun Bactericidal Concentration (MBC) were determined using the microdilution method proposed by Mann & Markham, 1997. The relationship between terpenes and penicillin were determined using a modification of the method proposed by Didry et al. (1995). The Fractionary Inhibitory Concentration (FIC) was determined. Results: Carvone, eugenol, geraniol and thymol were active against these microorganisms. The most active was thymol with a CIM of 7,5 mg/ml for S. aureus MSSA and 30,15mg/ml for MRSA and MBC=60,31mg/ml. Menthol, mentone, citronellol and mircene did not show any antibacterial activity. Carvone and penicillin were the mixture that showed a sinergistic effect, with a FIC of 0,077 for MRSA, considering synergistic the values below 0,5. Discussion: Carbona can be used together with penicillin increasing his action over strain multiresistant to antibiotics as S.aureus MRSA .