INVESTIGADORES
AGÜERO Tristan Horacio
congresos y reuniones científicas
Título:
cell signaling pathways involved in Xenopus laevis neural crest development
Autor/es:
MARCELA BONANO; TRISTÁN AGÜERO; JUAN P. FERNÁNDEZ; MANUEL J. AYBAR
Lugar:
Centro Cultural Rosario, Argentina
Reunión:
Congreso; XLII Reunión Anual SAIB; 2006
Resumen:
The neural crest (NC) develops at the border between neural plate and the prospective epidermis in all vertebrate embryos. NC cells are highly migratory and generate many derivatives (neurons, pigment cells, cartilage, endocrine cells, etc). It has been demonstrated that the signals BMP4, Wnt, FGF, Notch/Delta and RA are involved in the induction of this tissue. However, the participation of other signals in NC specification has not been established. In this work we analyzed the expression pattern of the components of Banded hedehog/Gli3 (Bhh/Gli3) and Enothelin1/Endothelin Receptor A (Edn1/Ednra) cell signaling pathways. We found out that Bhh and Ednra are expressed at the NC territory. In order to study the function of Bhh/Gli3 and Edn1/Ednra pathways in the NC development we have carried out conditional gain- and loss-of function approaches. The microinjection of Bhh and Ednra mRNA produced an increase in the expression of NC marker genes (FoxD3, Slug and Snail). On the other hand, the use of specific inhibitors of these signals, the microinjection of dominant negative constructs and morpholino antisense oligonucleotides against Bhh/Gli3 and Ednra caused a decrease in the expression of NC markers. Our results suggest that Bhh/Gli3 and Edn1/Ednra signaling pathways are required for the initial induction, maintenance of specification and migration of NC cells in Xenopus embryos.