INVESTIGADORES
AGÜERO Tristan Horacio
artículos
Título:
Maternal Dead-end 1 Promotes Translation of nanos1 Through Binding the eIF3 Complex
Autor/es:
TRISTÁN AGÜERO; ZHIGANG JIN; SANDIP CHORGHADE; AUINASH KALSOTRA; MARY LOU KING; JING YANG
Revista:
DEVELOPMENT
Editorial:
COMPANY OF BIOLOGISTS LTD
Referencias:
Lugar: Cambridge; Año: 2017
ISSN:
0950-1991
Resumen:
Primordial germ cells (PGCs) in the embryo are the exclusive progenitors of the gametes in the adult and their loss results in infertility. During early development, PGCs are exposed to numerous signals that specify somatic cell fates. To prevent somatic differentiation, PGCs must silence their genome transiently, a developmental process that requires Nanos activity during early development. However, it is unclear how nanos translation is regulated in early embryos. We report here that translation of nanos1 after fertilization requires Dead End1 (Dnd1), a vertebrate-specific germline RNA-binding protein. We provide evidence that Dnd1 protein, whose expression is low in oocytes, but increases dramatically after fertilization, directly interacts with the translational machinery and relieves the inhibitory function of eukaryotic initiation factor 3f, a repressive component in the 43S preinitiation complex. This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development.