Ipomoea carnea induces apoptosis in lymph node cells in guinea pig model

GARCÍA ENRIQUE N; AGUIRRE MARIA V; TODARO JUAN S; CHOLICH LUCIANA A

Buenos Aires

Congreso; XLVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL; 2016

Ipomoea carnea (Convolvulaceae) causes poisoning of goats, sheep and cattle in many tropical and subtropical countries. Swainsonine (SW) an alkaloid isolated from this plant, it is a powerful inhibitor of lysosomal α-mannosidase and the abnormal glycoprotein metabolism causes immunological dysfunction, neuropathy, aberrant embryogenesis and gastrointestinal alterations. Several studies have communicated that Swainsonine induces apoptosis in vivo and in vitro. Up to date, however, the effects of I. carnea on lymphoid tissues have not yet been examined. Thus, the aim of this study was to determine the frequency and the distribution of programmed cell death in mesenteric lymph nodes of guinea pig poisoned by I. carnea along 40 days of study. Experimental poisoning was achieved by feeding animals with ?pellets? prepared with milled leaves of I. carnea mixed with commercial crushed pellets for rodents. For in situ detection of DNA fragmentation in paraffin-embedded lymph node sections, the TUNEL method was performed using in situ Cell Death Detection Kit POD (Roche). The apoptotic index was expressed as the percentage of TUNEL-positive cells out of the total number of cells counted. TUNEL reaction stained mainly apoptotic nuclei of mesenteric lymph node cells. The pattern of labeling distribution of dead cells was different in each experimental group. The 40 days treated group showed high immunoreactivity in the germinal center of follicles, with a few TUNEL positive cells in the mantle zone. Few apoptotic cells were scattered distributed in the interfollicular zones. 15.57±5.31% apoptotic cell labeled of the 40 days I. carnea fed group, this value was significant statistical (P< p 0.0001) compared to the others experimental groups, the control and 20 days treated group with 3.00±1.58% and 5.80±1.10 % labeled apoptotic cells respectively. Our results evidence the enhancement of the programmed cell death of mesenteric lymph node cells induced by 40 days I. carnea ingestion.