a-manosidosis adquirida en cobayos por Ipomoea carnea.

CHOLICH LUCIANA A; GIMENO EDUARDO J.; ORTIZ MARIA L; GARCIA ENRIQUE N; TEIBLER GLADYS P; ACOSTA DE PEREZ OFELIA C

Esperanza

Jornada; XX Jornadas Nacionales y XV Latinoamericana de Farmaco - Toxicologia Veterinaria; 2010

Facultad de Veterinaria -UNL

Introduction: The intoxication of plants of the genera Swainsona, Oxytropis, Astragalus, and Ipomoea in ruminants produce lysosomal storage disease characterized by cytoplasmic vacuolation, mainly in neurons. Swainsonine and calystegines B1, B2 and C1 were the isolated toxic principles of I. carnea. Recently, we proposed the guinea pig as a new experimental model for the reproduction of neuronal storage disorders induced by this plant. The aim of the present work was to evaluate cerebellar lesions induced by Ipomoea carnea intoxication in guinea pigs. Material and Methods: Leaves of the plant were dried at 37ºC and milled. They were mixed homogeneously with commercial guinea pig pellets, previously hydrated with water for making ?small balls?. A mixture pellets-dry leaves in proportion 50:50 % were administered during 75 days. Clinical signs included hirsutism, emaciation and progressively loss of body weight. Samples of cerebellum were fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned at 5 µ µm thickness. Samples were stained with hematoxylin and eosin (H&E) and also with lectinhistochemistry. Results and Discussion: Histopathological evaluation of treated animals staining with H&E revealed the presence of small cytoplasmatic vacuoles in Purkinje cells and deep nuclei, these were strongly stained with WGA, s-WGA and LCA. The controls did not show any significant lesions. Conclusion: We conclude that Ipomoea carnea spp. fistulosa induces in guinea pigs similar lesions to other glycoprotein lysosomal storage diseases, mainly with the inherited genetic a-mannosidosis.