CHOLICH Luciana Andrea
congresos y reuniones científicas
Detection of ergot derivates from Ipomoea carnea responsible of addictive effect in intoxicated goats.
CHOLICH LUCIANA A; TORRES ANA M; CAMARGO FRANCISCO; RIOS ELVIO E; ACOSTA DE PEREZ OFELIA C
Mar del Plata
Congreso; XLII Reunion Anual de la Asociacion Argentina de Farmacologia Experimental.; 2010
Hotel 13 de Julio
Ipomoea carnea is a toxic plant, able to generate a poisoning in the goats, it is characterized by hepatic damage, nervous upheavals and death. The addiction in rumiant is commonly cited as a clinical sign of Ipomoea intoxication. The main objective of the present study was identify the possible alkaloid responsible for this preference. 1 g of the dry leaves were powdered and extracted by maceration with methanol for 72 h. Thereafter, the extracts were filtered under vacuum and concentred using a rotary evaporator. It was come to make different tests for the d-Lysergic acid detection. Such were fluorescence test (365 nm), chromatic test (Dragendorff and Van Urk-Erlich reagents) and thin-layer chromatographic (TLC) procedure based on the use of silica gel GF as adsorbent and chloroform-methanol (9:1) as eluent. The extract of dry leaves I. carnea was analized by TLC, this showed seven fluorescent spot at Rf of 0; 0,02; 0,03; 0,07; 0,10; 0,26; 0,41; 0,67 and 0,76. Three fluorescent blue spot at 365 nm with Rf of 0,02, 0,41 and 0,67 have been visualizad and gave a positive reaction with Van Urk reagent. The results demonstrated the prensence of derivates del ergot.