CHOLICH Luciana Andrea
congresos y reuniones científicas
INTOXICATION BY ALKALOIDS FROM Ipomoea carnea spp. fistulosa IN GUINEA PIGS: HISTOPATHOLOGICAL LESIONS ON PANCREAS AND KIDNEY
CHOLICH, LUCIANA A; GIMENO EDUARDO J. ; TEIBLER GLADYS P.; GARCIA DENEGRI MARIA E; ACOSTA DE PEREZ OFELIA C
Congreso; XL Reunion Anual de la Asociacion Argentina de Farmacologia Experimental; 2008
Facultad de Ciencias Veterinarias
Intoxication by Ipomoea carnea in ruminant is characterized by cytoplasmic vacuolation in cells of organs. Calystegines (0, 05%) and swainsonine (0, 02%) were isolated in this plant and produce inhibition of á and â galactosidases, á glucosidase and lysosomal á-manosidase respectively. The aim of this study was demonstrate the toxic effects of the alkaloids present in dry leaves of the plant administered to guinea pigs. Histological evaluation and lectin histochemistry was applied to identify stored specific sugars in cells. In the cytoplasm of exocrine pancreas and renal tubular cells was showed the presence of vacuoles and were identificated lectins such as Con-A (Concanavalia ensiformis), sWGA (Succinil triticum vulgaris), WGA (Triticum vulgaris) and LCA (Lens culinary). This result is coincident with the lectin histochemistry staining pattern of the vacuoles described for ruminant and also indicates that they contain N-glycosidically bound oligossacharides.