IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EFFECT OF DIETARY CAROTENOIDS ON THE ARGENTINE SHRIMP BROODSTOCK Pleoticus muelleri BATE
Autor/es:
FENUCCI, J.L.; PISANI, E; DÍAZ, A.C; VELURTAS, S. M
Lugar:
Seattle
Reunión:
Congreso; Aquaculture America 2014; 2014
Institución organizadora:
World Aquaculture Society
Resumen:
EFFECT OF DIETARY CAROTENOIDS ON THE ARGENTINE SHRIMP BROODSTOCK Pleoticus muelleri BATE Fenucci, J.L.*,Pisani, E., Díaz, A.C. and Velurtas, S.M. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CONICET, Funes 3350, B7602AYL, Mar del Plata, Argentina. jfenucci@mdp.edu.ar Pleoticus muelleri is the shrimp with highest commercial value found in cold temperate seas of Argentina and Brazil, and it is a potentially cultivable species. To carry out its cultivation, it is necessary to understand the processes of maturation and nutritional requirements to ensure profitable and long term sustainability. The use of bioactive substances as nutritional additives to improve crop yield penaeoids arises high attention and, in the case of carotenoids, studies are underway to define its biological function as a dietary supplement. The aim of this research was determine the effect of dietary carotenoids on shrimp broodstock. During 60 days immature females were kept in circular tanks with aeration system, 33 ups salinity, 18°C, pH 7, 11:14 h photoperiod). Two supplemented pelletized feeds were tested, one with 300 mg β-carotene and the other with 300 mg astaxanthin/kg diet, also a diet without carotenoids.was test as control. Female specimens of one day (wild) and 7 days of acclimatization (initials) were sampled. After 60 days of culture the ovaries, midgut gland and integument were sampled. Non polar (β-carotene) and polar (astaxanthin) carotenes were determined using UV-visible spectroscopy. Antioxidant activity was tested from midgut gland by magnetic resonance spectroscopy (EPR). Addition of carotenoids positively influenced ovarian development. 100% of the females fed both astaxanthin or β-carotene were mature, while in Control females only 75%. Ovaries of mature females exhibited the highest concentration of carotenoids, showing that these compounds act as intermediary precursors of highly bioactive molecules as retinoids. The abundance of free astaxanthin regarding total carotenes (Table 1), suggests that excess of free astaxanthin is biotransformed to esterified astaxanthin (integument stored). Either with or without addition of carotenes, the midgut gland presents a antioxidant protective capacity, evidenced by the ability to react with the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Fig. 1).