IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Maltase Activity in Mesenterial Filaments of the South-American Sea Anemone Bunodosoma zamponii
Autor/es:
DEL VALLE, J.C; ACUÑA,F.H. ; LÓPEZ MAÑANES, A,A.
Reunión:
Conferencia; 8th International Conference on Coelenterate Biology (ICCB); 2013
Resumen:
Knowledge of the digestive physiology of sea anemones at the biochemical level is scarce, particularly in South-American species. The final digestion of various dietary glycogenic carbohydrates depends on suitable levels of disaccharidases (ie: maltase). In spite of their important physiological role in maintaining glucose homeostasis, to the best of our knowledge, their are no studies on the occurrence of maltase activity in the coelenteron of sea anemones. The present objective was to determine the existence and biochemical characteristics of maltase activity in coelerenton mesenterial filaments of the South- American sea anemone Bunodosoma zamponii. Sea anemones (n=10) collected in the intertidal zone of Punta Cantera (Mar del Plata, Buenos Aires, Argentina) were maintained at 35 psu, 12 h L/12 h O and 20°C The supernatant (10000xg) from homogenate mesenteric filaments was used. Maltase activity was assayed by hydrolysis of 28.0 mM of maltose in 0.1 M maleate/NaOH buffer (pH 6.4) at 37°C. To study the effect of pH, maltose concentration, and temperature, the activity was determined at different pH levels (5.9-8.4), substrate concentrations (0.56-42 mM) and temperature (4-37°C) of the reaction mixture, respectively. Maltase activity was high over the wide range of pH and temperatures tested, and exhibited Michaelis-Menten kinetics (Km 0.31± 0.4 mM). Our results show the occurrence of maltase activity in mesenterial filaments of B. zamponii, suggesting its capacity for digestion and potential use of various glycogenic substrates as nutrient sources. The high activity over a wide range of pH and temperatures suggests that this capacity would continue to be maintained under varying physiological and/or environmental conditions.