IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
artículos
Título:
Long-Term Starvation and Posterior Feeding Effects on Biochemical and Physiological Responses of Midgut Gland of Cherax quadricarinatus Juveniles (Parastacidae)
Autor/es:
SACRISTÁN H.J.; FERNANDEZ GIMENEZ A.V.; ANSALDO M.; LÓPEZ GRECO L.S.; FRANCO-TADIC L.M.
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2016 vol. 11 p. 1 - 19
ISSN:
1932-6203
Resumen:
Crustaceans experience starvation periods during their growing process as a result of sequential molting. Starvation causes reallocation of energy resources and to have pro-oxidant effects. The aim of the present study was to determine the effect of long-term starvation and posterior feeding on energetic reserves, oxidative stress, digestive enzymes, and histology of the midgut gland in Cherax quadricarinatus. Feeding trials were carried out on juveniles crayfish (6.27 g initial weight) held in aquaria. Three treatments were tested in triplicate groups during 80 days. The control group (F) was daily fed with TetraColor granules (TETRA®). In the other two treatments, the starved animals were not fed until day 50, and thereafter half the animals were starved for remainder of the experimental period (Group Starved S) whereas the other half was fed up to day 80 [Group Starved-fed (SF)]. The midgut gland of each crayfish was dissected and used for measuring the reduced glutathione, lipids and glycogen levels. Also the organ was utilized for the assessment of soluble protein content, lipid peroxidation and protein oxidation levels, as well as catalase, lipase and proteinase activities. According to the results of the present study, when the redclaw crayfish C. quadricarinatus are long-term starved, it does not grow, reducing the digestive gland weight, presenting histological alteration in the midgut gland, using the glycogen and lipid reserves as source energy, reducing digestive lipase activity and reduced glutathione levels, and will not be altering the catalase activity. This study provides relevant biological information on physiological responses of crayfish under long-term starvation.