IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
artículos
Título:
Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine
Autor/es:
DÍAZ, A.O.; PLAUL S.E.; BARBEITO, C.G.; PASTOR R.
Revista:
MICROSCOPY RESEARCH AND TECHNIQUE.
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Lugar: New York; Año: 2016 vol. 79 p. 140 - 148
ISSN:
1059-910X
Resumen:
The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited for gas exchange. Instead, the cranial portion is a digestion and absorption site, as it has a typical intestinal epithelium with columnar cells arranged in a single row, villi and less vascularized tunica mucosa. Therefore, the intestine was studied by light and electron microscopy to assess differences between the cranial, middle and caudal portions. In order to characterize the potential for cell proliferation of this organ, we used anti-proliferating cell nuclear antigen antibody and anti-Na+K+-ATPase monoclonal antibody to detect the presence of Na+/K+ pump. In C. paleatus it was observed that cell dynamics showed a decreasing gradient of proliferation in cranio-caudal direction. Also, the intestine of this catfish is an important organ in ionoregulation: the basolateral Na+/K+ pump may have an active role, transporting Na+ out of the cell while helping to maintain the repose potential and to regulate cellular volume.