IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
artículos
Título:
The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
Autor/es:
CHIMEO NUÑEZ, CINDY; FERNÁNDEZ GIMENEZ, ANALIA VERÓNICA; MICHELLANGELO CAMPANELA; OFELIA MENDEZ-ROMERO; MUHLIA ALMAZAN, ADRIANA
Revista:
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
Editorial:
SPRINGER/PLENUM PUBLISHERS
Referencias:
Lugar: New York; Año: 2015 vol. 47 p. 383 - 393
ISSN:
0145-479X
Resumen:
Abstract The whiteleg shrimp species Litopenaeusvannamei is exposed to cyclic changes of the dissolved oxygenconcentration of seawater and must neutralize the adverseeffects of hypoxia by using ATP as energy source. In crustaceans,the mitochondrial FOF1-ATP synthase is pivotal to thehomeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebratesare equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, wereport two variants of the mitochondrial FOF1-ATPase InhibitoryFactor 1 (IF1) ubiquitously expressed across tissues ofthe Litopenaeus vannamei transcriptome: the IF1_Lv1 and theIF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp,encodes a 104 aa long protein and its mRNA amounts aresignificantly affected by hypoxia and re-oxygenation. TheIF1_Lv2, with a sequence of 654 bp, encodes instead for aprotein of 85 aa. Both proteins share a 69 % homology andcontain a conserved minimal inhibitory sequence (IATP domain)along with a G-rich region on their N-terminus typicalof the invertebrate. In light of this characterization IF1 is herediscussed as an adaptive mechanism evolved by this marinespecies to inhibit the FOF1-ATPase activity and avoid ATPdissipation to thrive in spite of the changes in oxygen tension.Keywords IF1, FoF1-ATPase . Hypoxia . Inhibitor .Transcriptional regulation . Shrimp