Integrative study at the biochemical level in a intertidal crab from Mar Chiquita coastal lagoon (Buenos Aires, Argentina) upon acute exposure to high zinc

LAURA PANELO; ANA M. PANZERI; JUANA C. DEL VALLE; ALEJANDRA A. LÓPEZ MAÑANES

Pan-American Journal of Aquatic Sciences

Pablo Muniz

Año: 2015 vol. 10 p. 55 - 67

1809-9009

Abstract In Mar Chiquita coastal lagoon (Argentina) acute high increases in Zinc (Zn) occurreaching  1.22  mg  x  L-1,  but  integrative  studies  on  possible  effects  at  thebiochemical-physiological  level  on conspicuous invertebrate species  inhabiting this  area arelacking.   We determined  body  and  vital  organs  mass,  hyper/  hypo-regulatory  capacity;Na+-K+ATPase  activity  alkaline  phosphatases  (AP)  activities  in  chela  muscle,  maltase  andsucrase activities in the hepatopancreas and glycemia of adult  males of the euryhaline crabNeohelice granulata upon exposure during 96 h to 1.22 mg x L-1 Zn in hyper- and hypo-osmoticmedia. Body, anterior and posterior gills, hepatopancreas and chela muscle mass and hyper andhypo-regulatory capacities were not affected suggesting maintenance of body and physiologicalcondition.  The  fact  that  upon  Zn  exposure  in  hypo-osmotic  media  Na+-KATPase  activitydecreased in posterior gills  while increased in anterior gills  along with increased glycemiasuggests the occurrence of biochemical compensatory adjustments.  Since no changes occurredin maltase and sucrase activities in the hepatopancreas or AP in chela muscle in hypo-osmoticmedia,  other  key  metabolic  parameters  appeared  not  to  be  comprised.  The  fact  that  inhyper-osmotic media Zn exposure no effects were found suggests the occurrence of specificeffects of Zn along upon a  hypo-osmotic challenge.+