Histopathologic alterations of the shrimp Pleoticus muelleri by effect of environmental nitrite.

ESPINO, M.L.; DÍAZ, A.C.; FENUCCI, J.L.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2014 vol. 38 p. 118 - 118

0327-9545

The aim of this work was to evaluate the effects of the nitrite on the histology of gills and hepatopancreas of P. muelleri. Shrimp were exposed to nitrite (0 to 1000mg/l) by 96hs. Histological techniques were performed on the cephalothorax of each individual. In the hepatopancreas, the changes were detected since 200mg/l of nitrite, consistent with an increase in the number of cells B and hyperplasia zones. Upper concentrations of nitrite caused distortion of the tubular structure and a high degree of cytoplasmic retraction in the cells. To concentration of 500mg/l, the cells showed abundant vacuolization (foamy cells) and only the F cells can be identified, to 1000mg/l all cells lost their cell identity. In the gills, the treatment with nitrite caused disorganization epithelial and folding of the cuticle, both effects were more prominent with the increase of this poluent. Upper concentrations of environmental nitrite caused distortion of the marginal channel, occurrence of subcuticular lagoons and collapse of the lamella. To 1000mg/l was detected disruption of the cuticle with the consequent deterioration of the gill lamella. In conclusion, the histological analysis of the hepatopancreas and gills of P. muelleri can be used as a biomarker by nitrite pollution.