IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
artículos
Título:
Effects of dietary carotenoids in the shrimp broodstock Artemesia longinaris.
Autor/es:
PISANI, E.; DÍAZ, A.C.; VELURTAS, S.M.; FENUCCI, J.L.
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Lugar: Mendoza; Año: 2014 vol. 38 p. 47 - 47
ISSN:
0327-9545
Resumen:
The aim of this study was to determine the effect of dietary carotenoids over bioacumulative capacity of carotenoids in different organs, total antioxidant activity, growth and maturation. Immature females (with unilateral eyestalk ablation) were held in 3m diameter tanks with aeration (33? salinity, 18°C, pH 7, 12:12 h photoperiod). Three diets were tested, two supplemented with β-carotene or astaxanthin (300mg carotene/kg diet) and other without supplementation. After 60 days ovaries, midgut gland and integument were removed. Nonpolar carotene (β-carotene) and polar (astaxanthin) was determinate by using UV-visible spectroscopy. Antioxidant activity of midgut gland was analyzed by magnetic resonance spectroscopy. In all treatments 100% of females have ripen ovaries. Carotenoids enhanced weight gain in both treatments related to the control. Both, the integument and ovary showed higher bioaccumulation of β-carotene than free astaxanthin. Carotenoids were not detected in the midgut gland. Either with or without addition of carotene, the midgut gland exhibited an antioxidant protection, evidenced by the ability to react with the stable radical 2,2-diphenyl-1-picrylhydrazyl.