Characterization of glycoconjugates in the pharyngeal cavity and the oesophagus of Odontesthes bonariensis by lectins.

DÍAZ, A.O.; M.F. TANO DE LA HOZ; A.M. GARCÍA; A.H. ESCALANTE & A.L. GOLDEMBERG

BIOTEMAS

Universidad Federal de Santa Catalina

Lugar: Santa Catalina; Año: 2012 vol. 25 p. 157 - 163

0103-1643

Seven types of biotinylated lectins were used to examine glycoconjugates (GCs) expression patterns in the mucosa of the pharyngeal cavity and the oesophagus of the Odontesthes bonariensis. Con-A, RCA-I and WGA binding sites were positive for glycocalyx, epithelial cells and mucous cells in both organs with similar reaction patterns. PNA just stained glycocalyx and epithelial cells in the mucosa in both organs. DBA, SBA and UEA-I stained only the oesophagus, and a greater affinity to glycocalyx and the epithelial cells was observed. The results of this study were discussed and their possible functional implications were compared.Odontesthes bonariensis. Con-A, RCA-I and WGA binding sites were positive for glycocalyx, epithelial cells and mucous cells in both organs with similar reaction patterns. PNA just stained glycocalyx and epithelial cells in the mucosa in both organs. DBA, SBA and UEA-I stained only the oesophagus, and a greater affinity to glycocalyx and the epithelial cells was observed. The results of this study were discussed and their possible functional implications were compared.