INVESTIGADORES
CERUTI Julieta Maria
congresos y reuniones científicas
Título:
PROTEIN MALNUTRITION DIMINISHES CEREBRAL CORTEX BDNF EXPRESSION AND EVOKES ANXIETY LIKE BEHAVIOURS
Autor/es:
BELLUSCIO, LM; BERARDINO, BG; CERUTI, JM; CÁNEPA, ET
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la SAIB; 2012
Institución organizadora:
SAIB
Resumen:
ABSTRACT
PROTEIN MALNUTRITION DIMINISHES CEREBRAL
CORTEX BDNF EXPRESSION AND EVOKES ANXIETYLIKE
BEHAVIOURS
Belluscio LM, BerardinoBG, Ceruti JM, Cánepa ET.
Laboratorio de Biología Molecular, Departamento Química
Biológica, FCEN, UBA, Ciudad de Buenos Aires
Undernutrition and malnutrition continue to be some of the most
common conditions that affect the development of children living
in developing countries. The aim of this work was to study the
effects of early protein malnutrition over the development of the
pup nervous system.
Pregnant CF-1 mice were fed with normal or low protein diet
during pregnancy and lactation. Several developmental milestones
were analyzed from P1 to P21. Pups from the low protein group
exhibited a delay in righting reflex and grip strength progress, and
in eye and ear opening and fur growth.We studied the expression of
several neurotrophins and immediate early genes by RT-PCR and
western blot and found a reduction in BDNF expression in the
cortex of 3 week old pups from the low protein group. The presence
of chromatin modifications was assessed by western blot, and we
found an increase in the presence of acetylated histone 4 in the
cortex of malnourished pups. Pups from both groups were fed with
standard lab chow after weaning and subjected to the elevated plus
maze (EPM) and open field tests in order to study the presence of
anxiety-like behaviours in the young adults. The offspring from
low protein group exhibited a more anxious behaviour in the EPM.
Our results indicate that pups subjected to early protein
malnutrition suffered a developmental delay and presented a more
anxious behaviour.