Effects of static magnetic fields on nicotinic cholinergic receptor function

TOLOSA F; BOUZAT C * (ULTIMA AUTORIA COMPARTIDA); CRAVERO W

BIOELECTROMAGNETICS.

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2011 vol. 32 p. 434 - 442

0197-8462

Ligand-gated ion channel kinetics were studied in mammalian transfected cells encoding adult mouse muscle acetylcholine (ACh) receptors. We measured macroscopic and single-channel currents using the outside-out and cell-attached patch-clamp configurations. Cultured cells were exposed to moderate intensity inhomogeneous static magnetic fields up to 180 mT and measurements were performed for temperatures ranging from 5 to 50 °C. We found no significant changes in ACh-elicited macroscopic or single-channel currents. We observed the expected dependence in current decay constants with temperature, but negligible magnetic field influence on the channel?s kinetics.