Antiosteolitic effect of melatonin on male rats treated with glucocorticoids. Statistical longitudinal and transversal studies

LADIZESKY MG; MORENO AYALA MA; MASTAGLIA S ; BOGGIO V; SCCACHI PA

Otro; XXIV Reunión Anual de la Asociación Argentina de Osteología y Metabolismo Mineral; 2007

The present study was undertaken to examine the effect ofmelatonin (MEL) (in drinking water, about 500 ìg/day) onlong daily treatment of male rats with methylprednisolone(CORT) (5 mg/kg i.p.). Animals were treated during 75 dayswith MEL and/or CORT. We work with 5 groups of animals(10–14 p/group) – CONTROL, CORT, MEL, MEL+CORTand MEL before CORT. The following parameters were mea-sured at days – Basal, 15, 30, 45, 60 and 75 after the beginningof the treatment – Body weight, calcemia (CaS), phosphatemia(PS), serum bone alkaline phosphatase activity (FAO) and CTXin serum. We work with Statisticals Studies – Longitudinalalong the experiment (GEE – generalized equation estimation)and Transversal (ANOVA), at day 45. Results – (1) CORTgroup presents higher levels of calcemia than MEL group(pb0.013). (2) After 35 days of treatment with CORT, MELenhanced FAO (bone formation) (pb0.05). (3) MEL decreasedPS levels in CORT group (pb0.011). (4) MEL reduced CTXlevels (bone resorption) shown by CORT group (pb0.021). (5)MEL reduced body weight and diminished the incrementedvalues induced by CORT (pb0.02). Summary – MEL iscapable of inhibit and/or decrease bone resorption induced by75 days of administration of methylprednisolone in male rats.