INVESTIGADORES
PONCE Nora Marta Andrea
artículos
Título:
Commercial cell wall hydrolytic enzymes for producing pectin enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)
Autor/es:
FISSORE, E.N; PONCE, N.M.A; WIDER, E. A; STORTZ, C.A; GERSCHENSON, L.N; ROJAS, A.M
Revista:
JOURNAL OF FOOD ENGINEERING
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2009 vol. 93 p. 293 - 301
ISSN:
0260-8774
Resumen:
A cell wall-enriched butternut (Cucurbita moschata Duch ex Poiret) powder was obtained by drying mesocarpwastes; this powder was used as the substrate for commercial cellulase (A-product) or hemicellulase(B-product) action. Yields were 4.70% and 6.12%, respectively. Both pectin-enriched products were watersoluble fibers and only the latter showed important thickening effect at 20 g dm3-concentration inwater. This product was a highly methylated (methylation degree, DM, 72.6) pectin with 45.2 g/100 gof galacturonic acid and 11 g/100 g of neutral sugars. Cellulase produced intense hydrolysis leading toa less methylated (DM: 54.2) A-fraction. It was observed that not only average molecular weight, but alsothe molecular structure derived from chemical composition affected flow behavior and viscosity enhancingcapacity. As A-fraction was enriched in rhamnogalacturonan I, probably, rhamnose kinks wereresponsible for the poor thickening effect at 20 g dm3-concentration in water, in spite of its homogeneousmolecular weight profile, which showed only one peak at 793,000. This fraction presented the lowestdensity of macromolecular interactions in water, behaving as a diluted hydrocolloid solution asconfirmed by its fitting to the Cox–Merz rule. Both isolated soluble fractions produced a delay in the glucosedialysis assay.