Overexpression of the carbohydrate binding module from Solanum lycopersicum Expansin 1 (Sl-EXP1) modifies tomato fruit firmness and Botrytis cinerea susceptibility
PERINI, MAURO; SIN, IGNACIO; VILLARREAL, NATALIA MARINA; MARINA, MARÍA; POWELL, ANN; MARTINEZ, GUSTAVO; CIVELLO, PEDRO MARCOS
PLANT PHYSIOLOGY AND BIOCHEMISTRY
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Lugar: Paris; Año: 2017 p. 122 - 122
Firmness, one of the major determinants of postharvest quality and shelf life of fruits, is determined mainly by the mechanical resistance imposed by the plant cell wall. Several strategies have been pursued to control the loss of fruit firmness during storage. Most of the approaches have been to suppress the expression of key enzymes involved in the cell wall metabolism. Expansins are proteins involved in non-hydrolytic plant cell wall metabolism, especially for essential processes where relaxation of the cell wall is necessary, such as during fruit development and ripening. Expansin proteins have a characteristic putative catalytic domain and one carbohydrate-binding module (CBM).In this report we analyze the possibility that overexpressing the carbohydrate binding module of a fruit-specific expansin is appropriate for developing a general strategy to control cell wall degradation. Transgenic tomato lines constitutively overexpressing the carbohydrate binding module of Solanum lycopersicum expansin 1 (CBM-SlExp1) in the cell wall were generated and the phenotypes of the plants and fruit analyzed. Overexpression of CBM-SlExp1increased the mechanical resistance of leaves, resulted in firmer fruits with delayed softening, and reduced the susceptibility of fruit to infections by Botrytis cinerea.