INVESTIGADORES
DELPINO Maria Victoria
congresos y reuniones científicas
Título:
Type 4 secretion system (T4SS) and the secreted protein BPE005 from Brucella abortus modulates hepatic stellate cells responses
Autor/es:
PAULA CONSTANZA ARRIOLA BENITEZ; AYELÉN IVANA PESCE VIGLIETTI; DIEGO REY SERANTES; CLAUDIA KARINA HERRMANN; SILVIA VANZULLI; DIEGO JOSÉ COMERCI; GUILLERMO HERNÁN GIAMBARTOLOMEI; MARIA VICTORIA DELPINO
Reunión:
Congreso; 56. LXIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI). 18-21 Noviembre de 2015.; 2015
Resumen:
Type 4 secretion system (T4SS) and the secreted protein BPE005 fromBrucella abortus modulates hepatic stellate cells responses Paula Constanza Arriola Benitez1, Ayelén IvanaPesce Viglietti1, Diego Rey Serantes2, Claudia Karina Herrmann2, Silvia Vanzulli3, Diego JoséComerci2, Guillermo Hernán Giambartolomei1, Maria Victoria Delpino1 1 Instituto de Inmunología, Genética y Metabolismo (INIGEM)-CONICET-UBA 2 Instituto de Investigaciones Biotecnológicas (IIB)?CONICET-UNSAM 3 Academia Nacional de Medicina (IMEX)-CONICET Abstract: The liver is affected in human brucellosis. Hepatic stellatecells (HSC) are the major cells involved in liver fibrosis, and they are theresident antigen-presenting cells. B. abortus (Ba) could be involved in themodulation of immune response in liver through activation of inflammasome withconcomitant fibrosis to repair the damage. Ba tilts HSC to a profibrogenic phenotypein a way that involves a functional T4SS and the secreted protein BPE005. Todetermine in vivo relevance of the role of BPE005 in liver fibrosis, BALB/cmice were infected with Ba and bpe005 mutant. Masson's trichrome (p<0.05)and Sirius red (p<0.01) staining revealed that the presence of fibroticpatches was lower in mice infected with Ba bpe005 mutant than in those infectedwith Ba. TGF-β1 secretion was lower in liversfrom Ba bpe005 mutant-infected mice than those infected with Ba (p<0.05),and HSC are the main cells involved in TGF-β1 secretion as wasrevealed by immunohistochemistry. HSC could express inflammasomes, our resultsusing LX-2 cell line indicated that Ba induce the secretion of IL-1β in a mechanism dependent on the presence of afunctional T4SS but not the BPE005 protein (p<0.001). Experiments conductedto determine if Ba could modulate antigen presenting functions of LX-2,revealed that Ba infection induced MHC-I (p<0.01) and MHC-II (p<0.001)expression, but not affect the expression of CD40, CD80 and CD86 in LX-2. Theseresults indicate that the T4SS and BPE005 are involved in most of the mechanismsinvolved in the modulation of liver responses.