INVESTIGADORES
DELPINO Maria Victoria
congresos y reuniones científicas
Título:
The protein moiety of the Brucella Omp19 lipoprotein, co-administered as oral adjuvant, induces a Th1 immune response.
Autor/es:
IBAÑEZ ANDRES; CORIA LORENA; PASQUEVICH KARINA; BARRIONUEVO PAULA; DELPINO M VICTORIA; GIAMBARTOLOMEI GUILLERMO; CASSATARO JULIANA
Reunión:
Congreso; 28. Malnutrition, Gut-Microbial Interactions and Mucosal Immunity to Vaccines (T1). Keystone Symposia, 7- 11 november. New Delhi, India.; 2011
Resumen:
Previous results demonstrate that
the protein moiety of the Brucella Omp19
lipoprotein (U-Omp19) administered by the oral route has self-adjuvant
properties. In this study we evaluated its mucosal adjuvant capacity using as model
antigen (Ag) ovalbumin (OVA). For this purpose mice were immunized orally with
OVA, U-Omp19+OVA, or choleric toxin (CT)+OVA. By flow cytometry we determined
that the percentage of CD4+ and CD8+ T cells expressing
α4β7 integrin increased in those mesenteric lymph nodes from U-Omp19+OVA in
comparison with OVA immunized mice. We also observed a significant DTH response
to OVA injection in U-Omp19+OVA while OVA group did not.
Similarly, we observed that mice
immunized with U-Omp19+OVA induced significant production of IFN-γ in response to
OVA. By flow cytometry we determined that the percentage of CD4+ and
CD8+ T cells producing intracellular
IFN-γ -in response to OVA- increased in U-Omp19+OVA in comparison with CT+OVA
and OVA vaccinated
mice. We also observed that U-Omp19 immunization induces
Ag internalization by CD11b+ cells and the recruitment of dendritic cells (DCs)
CD11c+CD8+. In
conclusion, U-Omp19 as oral adjuvant induces the migration of CD4+ and CD8+ T
cells to gastrointestinal lamina propria, T cells responses in vivo and CD4+
and CD8+ T cells that produce IFN-γ in response to OVA, and the recruitment of
DCs wich are involved in the initiation of adaptative immune response.
This work was supported by grants from: Bill
& Melinda Gates Foundation through the Grand Challenges Explorations
Initiative, from the Agencia Nacional de Promocion Cientifica y Tecnologica
(ANPCyT-Argentina) and from University of Buenos Aires.