INVESTIGADORES
ALBARRACIN Virginia Helena
congresos y reuniones científicas
Título:
Aislamiento y selección de cepas de actinomycetes resistentes a cobre.
Autor/es:
ALBARRACÍN, V.H.; DEBES, M.A.; SALES, A.M.; AMOROSO, M.J.; ABATE, C.M.
Lugar:
Tucumán, Argentina
Reunión:
Jornada; XX JORNADAS CIENTÍFICAS DE LA SOCIEDAD DE BIOLOGÍA de TUCUMAN; 2003
Institución organizadora:
SOCIEDAD DE BIOLOGÍA de TUCUMAN
Resumen:
Copper is essential to the cell, but it becomes toxic in great concentration. Mining activities are responsible of the release of effluents containing great concentrations of copper directly to soils and water streams. Microorganisms living there support a selective pressure, that lead them to develop mechanisms to resist these conditions. The objective of this work is the isolation and selection of copper resistant actinomycetes strains that present the ability of sequestering copper from the medium to be used in future soil bioremediation strategies. In addition, the isolates were characterized by restriction analysis of ADNr 16s product. Nine actinomycetes strains were isolated from copper polluted soils samples. Qualitative and semiquantitative tests (solid medium) indicated that all of them but AB2C were high copper resistant (up to 1000 mg/L) Cuantitative test (liquid medium) indicates that the most resistant strains (AB2B, AB5A, AB5B Y AB5D) were inhibited only in  35 -17 % respect to the control. Copper residual determination shew that its declining in the liquid medium was 40 -50% respect to the initial copper concentration. ARDRA analysis indicated that all strains belong to the genus Streptomyces.