INVESTIGADORES
ALBARRACIN Virginia Helena
congresos y reuniones científicas
Título:
Caracterización de la capacidad acumuladora de cobre por una cepa de actinomycete con potencial uso en biorremediación.
Autor/es:
ALBARRACÍN V. H.,; SIÑERIZ LOUIS, M.; AVILA, A.; RODRÍGUEZ, H. C.; VIERA VIGO, M.C. ,; CUELLAR, A.L.; TABOADA, M. A.; ABATE, C. M.
Lugar:
Tucumán, Argentina
Reunión:
Jornada; XXII JORNADAS CIENTÍFICAS DE LA SOCIEDAD DE BIOLOGÍA DE TUCUMÁN; 2005
Institución organizadora:
SOCIEDAD DE BIOLOGÍA DE TUCUMÁN
Resumen:
Actinomycete strain AB0 was isolated from sediments of a copper polluted drainage channel. This strain was capable of growing in copper suplemented synthetic media, soil extract and soil. In all cases, copper acumulation was demonstrated by a reduction of residual copper in the medium and by the presence of intracelullar copper in subcelullar fractioning assays. The aim of this work is to search for proteins responsible for the citosol copper accumulation capacity observed in AB0 strain. On the other hand, a screening for plasmids is performed using different techniques, in order to determine if the nature of this copper accumulation capacity is cromosomal or plasmidic. Gel filtration cromathography coupled with atomic absortion spectrometry identified specific intracelullar copper associated proteins. Plasmids were no detected in AB0 strain using alcalyne lysis method for screening of regular plasmids and pulsed field gel electroforesis for megaplasmids. This could indicate that the copper accumulation related genes are chromosomally located