INVESTIGADORES
ALBARRACIN Virginia Helena
congresos y reuniones científicas
Título:
Extremophilic Actinobacteria from High Altitude Andean Lakes at the Argentinean Puna: Diversity and bioactivity assays
Autor/es:
FERRER M.G.; RASUK M.C.; MORENO J.R.; RODRIGUEZ E.; GÄRTNER W.; FARIAS M.E.; ALBARRACÍN V.H.
Lugar:
Puerto Vallarta
Reunión:
Simposio; 16TH INTERNATIONAL SYMPOSIUM ON THE BIOLOGY OF ACTINOMYCETES; 2011
Institución organizadora:
ISBA Board
Resumen:
High-Altitude Andean Lakes (HAAL) at the Argentinian Puna-High Andes represents an almost unexplored ecosystem of shallow lakes at altitudes from 3,000 to 6,000 m. Although exposed to extreme conditions (high UV irradiation, hypersalinity, drastic temperature changes, desiccation, high pH and volcanic activities), an outstanding microbial biodiversity has developed. Extremophilic actinobacteria constitutes an important group within these microbial communities which represent a promising source in the search for novel natural products; we herein investigated the diversity and preliminary bioactivity assays of actinobacterial strains isolated from different lakes at the HAAL. By using diverse selective isolation methods we obtain more than 120 actinobacterial strains from shallow water, sediments, microbial mats, stromatolites and flamingo fecal samples at 15 different lakes. Taxonomical identification was performed using phenotypic and genotypic data (i.e. Rep and Box-PCR, ITS, 16s rDNA and GyrB molecular markers). The genera represented in our collection were Streptomyces, but also rare actinobacteria as Kocuria, Brachybacterium, Microccocus, Microbacterium, Nesterenkonia, Rhodoccocus, Nocardia, Agroccocus, Dietzia, Arthrobacter and Brevibacterium. These strains were tested for citotoxicity activity against Mycobacterium smegmatis, Escherichia coli, Enteroccocus faecalis, Sacharomyces cerevisae, Candida albicans, Microccocus luteus and Staphyloccocus aureus. All strains tested revealed bioactivity against at least one of the model strains. This study confirms the significant biodiversity of extremophilic actinobacteria in the HAAL, and their potential for producing bioactive compounds.