In vitro anti-tumoral and immunomodulatory effects of acidic polysaccharides isolated from the fungus Gloeosoma mirabile

SOLANGE TORRES; MARIO RAJCHENBERG; MARÍA BELÉN PILDAIN; JOSÉ BECERRA; CLAUDIA PÉREZ

NATURAL PRODUCT RESEARCH

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2024

1478-6419

The acidic polysaccharides from Aleurodiscus mirabilis (APsAM) were extractedusing a hot water process and purified by N-cetylpyridinium bromide precipitation. TheAPsAM were characterized by Fourier transform infrared spectroscopy and showedcharacteristic bands of acidic polysaccharides (Aps). The gas chromatography–massspectrometry (GC-MS) analysis revealed the presence of different monosaccharides, mainlyconstituted by Arabinose (38.4%), followed by α-D-Galactopyranose (10%) and Methyl-β-D-galactopyranoside (9.4%). Further, immunomodulatory activities of APsAM onmacrophage RAW 264.7 cells were investigated. APsAM showed a dose-response effectfor RAW 264.7 cytotoxicity and secretion of IL-6. The APsAM showed an antitumoractivity by inhibiting breast (MCF-7), colon (HCT-116) and leukemia tumoral cell (U-937)proliferation with an IC 50 of 65.69 μg mL −1 , IC 50 of 15.50 μg mL −1 and IC 50 of 121.54 μgmL −1 , respectively. In the cell cycle analysis by flow cytometry showed that HL-60 cells inthe presence of the highest studied concentrations of APsAM (1,200 µg mL -1 ) inducesapoptosis in an 83%. These results indicate that APsAM-find promising opportunities forcancer treatment as a potential immunotherapeutic agent. Subsequently, thecharacterization and in vitro biological activity of polysaccharides from A. mirabilis hadbeen investigated to assess the potential applications as functional foods or nutraceutical.