Effect of Yerba Mate (Ilex paraguariensis) on Osteocytic Cells

D`ANDREA F; SANZ N; LOMBARTE M; RICO MJ; ROZADOS VR; SCHAROVSKY OG; DI LORETO VE; PLOTKIN LI; BRUN LR

Congreso; 2020 ASBMR Annual Meeting; 2020

American Society for Bone and Mineral Research (ASBMR)

Yerba mate (YM) infusion is frequently drank in several Latin American countries. Several active phytochemicals including caffeine and polyphenols have been identified in YM solution. Whereas caffeine has shown a negative impact on BMD in healthy postmenopausal women in particular when it is associated with low calcium diet, polyphenols have shown positive bone effects due to their antioxidant properties. In addition, we found that YM significantly increased MC3T3E1 osteoblastic cells viability in vitro and positive effect on BMD, trabecular bone biomechanical properties, and alkaline phosphatase levels in vivo. However, whether the infusion also affects osteocytes is not known. To examine this possibility, we evaluate the effect of YM components on MLO-Y4 osteocytic cells in vitro. Cell viability (Cell Proliferation Reagent WST-1, Roche) was evaluated before exposing cells (48 hours) to YM components (caffeine and two polyphenols: rutin and chlorogenic acid) in two different experiments: 1. caffeine (C 0.66, 1.66 and 3.33 µg/ml), chlorogenic acid (AC 1, 5 and 10 µg/ml) and their combinations (n=8/group). 2. caffeine (C 0.66, 1.66 and 3.33 µg/ml), rutin (R 1, 5 and 10 µg/ml) and their combinations (n=8/group). A qualitative analysis of the cell morphology according to treatment was made. Differences between groups were analyzed using one-way ANOVA test and it was considered significant if p