INVESTIGADORES
BRUN Lucas Ricardo Martin
congresos y reuniones científicas
Título:
Effects of Yerba Mate (Ilex paraguariensis) with Different Antioxidant Capacity on Bone and Oxidative Stress Parameters
Autor/es:
BRUN LR; VILLARREAL L; LOMBARTE M; D´ANDREA FE; DI LORETO VE
Lugar:
Orlando
Reunión:
Congreso; 2019 ASBMR Meeting; 2019
Institución organizadora:
ASBMR
Resumen:
Yerba mate (YM) infusion is frequently drank in several Latin Americancountries and contain multiples active phytochemicals as methylxanthines andpolyphenols. Previuosly we found in vivopositive effect on BMD, trabecular bone volume and in vitro osteoblastic cells viability (MC3T3E1) after treatmentwith YM components (caffeine, chlorogenic acid and rutin). Aim: evaluate the effect of YM with different antioxidantcapacity on bone tissue and oxidative stress parameters. Material and methods:30-day Sprague Dawley rats were divided into 3 groups (n=9/group) whichreceived ad libitum during 90 days:Control (water), YM,  YM+. The higherantioxidant capacity of YM+ was verified through the percentage of inhibitionof the radical 2,2-diphenyl-1-picrylhydrazyl and polyphenols content. After 90days, the total BMD was performed on tibias by DXA (Hologic). The morphologicalanalysis were performed on cross sections (500 μm in thickness) using asoftware (Image J 1.40). Cortical bone strength at the femoral mid-diaphysiswas determined using a 3-point bending test and trabecular bone strength wasevaluated by a compression test on thoracic vertebrae. The activity of alkalinephosphatase (AP) and oxidative stress parameters (glutathione peroxidase [GPx],catalase and lipoperoxidation) were measure in plasma. Results: BMD weresignificantly increased in both YM groups (YM= 10.1% andYM+= 9.1% vs control) without differences between them. The compressiontest on thoracic vertebrae was found significantly increase in both YM groups (YM=14.1% and YM+= 15.4% vs control) without differences between them. Nodifferences  in 3-point bending test and corticalmorphological parameters were found among groups. The oxidative stressparameters were found decrease in YM groups vs controls, without differencesbetween the YM groups: GPx (µmol/min.mg prot): control=0.014±0.001, YM= 0.011±0.001*, YM+=0.011±0.001* (*p<0.05 vs control); catalase(umol H2O2/min.mg prot): control= 0.30±0.25, YM= 0.15±0.08, YM+=0.12±0.05(p=0.0592); lipoperoxidation (uM equiv 1,2 MDA-TBA): control= 42.70±16.42, YM=21.07±11.77*, YM+=19.35±17.33* (*p<0.05 vs control). The AP wassignificantly increased in YM groups. Conclusion: both YM infusions showed apositive effect on BMD, trabecular bone biomechanical properties and AP levelswhereas reduced oxidative stress parameters in plasma without differences amongthem. The bone effect of YM could be due to a decrease of oxidative stress.