Effects of yerba mate (ilex paraguariensis) on bone and oxidative stress parameters

VILLARREAL L; ANSALDI M; D´ANDREA FE; LOMBARTE M; DI LORETO VE; BRUN LR

Mar del Plata

Congreso; LXIII Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Sociedad Argentina de Investigación Clínica (SAIC)

Yerba mate infusion(YM) intake is very common in South America. Higher bonemineral density (BMD) were found in rats and postmenopausal women who drank YM vs controls. Anassociation was described between bone loss with age and oxidative stress. Theaim of this study was to evaluate the effect of YM with different antioxidantcapacity on bone tissue and oxidative stress parameters. 30-day Sprague Dawleyrats were divided into 3 groups (n=9/group) which received ad libitum during 90 days: Control (water), YM (25 g/500 ml, 70°C),YM+ (YM 50 g/500 ml, 90°C). The highest antioxidant capacity of YM+ wasverified through the percentage of inhibition of the radical2,2-diphenyl-1-picrylhydrazyl (DPPH) and the content of total polyphenols. After90 days, the total BMD was performed on tibias by dual-energy X-ray absorptiometry(DXA, Hologic). Themorphological analysis were performed on cross sections (500 μm in thickness) using a software(ImageJ 1.40 - NIH).In addition, oxidative stress parameters: glutathione peroxidase (GPx),catalase and lipoperoxidation were measure in plasma. Results:As expected, no difference in body weight, food and drink intakewere observed. In addition, no differences in tibias length and corticalmorphological parameters were found. BMD were significantly increased in bothYM groups (YM= 10.1% and YM+= 9.1% vs control) without differences betweenthem. The oxidative stress parameters were found decrease in YM groups vscontrols, without differences between the YM groups: GPx (µmol.min-1.mgof prot-1): control= 0.014±0.001, YM=0.011±0.001*, YM+=0.011±0.001* (ANOVA, *p<0.05 vs control); catalase (umolH2O2 consumidos/min.mg prot): control= 0.30±0.25, YM= 0.15±0.08, YM+=0.12±0.05(ANOVA, p=0.0592); lipoperoxidation (uM equivalentes 1,2 MDA-TBA): control=42.70±16.42, YM= 21.07±11.77*, YM+=19.35±17.33* (ANOVA, *p<0.05 vs control).In conclusion, YM infusion showed a positive effect on BMD without differencesbetween its antioxidant capacity. This effect could be dueto lowoxidative stress in YM groups.