Effect of yerba mate (Ilex paraguariensis) on the restoration of ethanol-induced gastric mucosa damage in rats.

SALVATELLI A; PETRONI E; CORBO ML; LOMBARTE M; BRUN LR; DI LORETO VE

Congreso; Reunión Conjunta SAIC. SAI. AAFE. NANOMED-AR 2021; 2021

SAIC. SAI. AAFE. NANOMED-AR

There is a popular belief that the consumption of yerba mate (YM) causes symptoms of gastric distress (heartburn or gastritis). However, there is no scientific evidence in this regard. In previous experiments, we have evaluated the effect of chronic consumption of YM infusions on the gastric mucosa without finding signs of damage. We wonder how the previous state of the mucosa would influence the referred symptoms. The objective of this work was to study the effect of the ingestion of an infusion of YM on the restoration of the gastric mucosa of rats with a previous gastric injury due to ethanol consumption.Thirty-two fasted rats were administered 1 mL of 60% ethanol by orogastric tube to produce damage and then a 10% ethanol solution was supplied ad libitum (E). In addition, a group of control animals (C) received only water and another, YM infusion (25 g/500 ml, 70 °C, 5 min), (n = 8 each). After 7 days, animals were sacrificed (n = 8 each). E was discontinued in the remaining animals of group E (n = 24), they were separated and given water (E+W) or YM (E+YM). Euthanasia was performed at day 3 and 7, the stomachs were processed for histological evaluation of macro and microscopic lesions and the levels of lipoperoxidation were determined in homogenates of the mucosa.Histopathological analysis: C and YM showed a mucosa with normal characteristics both macro and microscopically. Group E evidenced loss of folds, edema and congestion. Microscopically, it presented slight distortion of the glandular lumens, disruption of the epithelial surface and mild edema and congestion. After the suspension of E, both E+W and E+YM macroscopically showed a mucosa with normal characteristics both at day 3 and 7. Microscopically, at day 3, both groups presented mild edema and congestion, and completely normalizing after day 7 of suspension of E. Evaluation of oxidative damage (mean ± SD): C: 0.26 ± 0.04 nmolTBARS / mg protein, YM: 0.17±0.05*, E:0.35±0.09, ANOVA, Tuckey post test, *p