INVESTIGADORES
STENGLEIN Sebastian Alberto
artículos
Título:
New media for increasing sporulation and germination of Phaeoisariopsis griseola conidia
Autor/es:
STENGLEIN S.A., AULICINO M., ARAMBARRI A.M. & BALATTI P.A.
Revista:
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Editorial:
SPRINGER
Referencias:
Año: 2006 vol. 115 p. 173 - 180
ISSN:
0929-1873
Resumen:
The slow growth rate of the fungus Phaeoisariopsis griseola and the availability of a homogeneous highly concentrated inoculum is an important constraint for pathogenicity or virulence studies, where plant inoculations are needed. Therefore, the purpose of this paper was to evaluate the effect, supplementation of culture media with Amaranthus cruentus seed meal, has on fungal growth and sporulation of isolates of P. griseola belonging to the Mesoamerican and Andean group. The amendment of PDA or V8 media with A. cruentus seed meal resulted in a considerable increase in the number of conidia and also in their capacity to germinate, which seems to depend mostly on the stage of maturity of conidia. It seems that either one or several of the nutritional factors provided by A. cruentus contributed to accelerate the production of conidia and their development, resulting in faster development of the disease and an earlier appearance of symptoms. Therefore, for cultural studies, especially for inoculum production and for pathogenicity evaluations, supplementation of the media with A. cruentus seed meal proved to be a good alternative.