INVESTIGADORES
MOLLERACH Marta Eugenia
congresos y reuniones científicas
Título:
Distribution of the Antiseptic Resistance Genes in Staphylococcus aureus Genomes from Latin American Countries.
Autor/es:
DI GREGORIO S; HAIM MS; MOLLERACH M
Reunión:
Congreso; World Microbe Forum. ASM & FEMS. Virtual-Global. 2021; 2021
Institución organizadora:
American Society for Microbiology and Federation of European Microbiological Societies
Resumen:
BACKGROUND: The increase in antiseptics use over the years has selected S. aureus strains that show decreased susceptibility to these compounds. This phenotype is associated with the presence of plasmidic qac genes encoding specific efflux pumps. Despite the concern about the possible role of antiseptics in the emergence/selection of multidrug resistant high-risk clones, data on S. aureus recovered in Latin America (LA) are limited.The aim of this study is to perform bioinformatic analyses to detect qac genes in publicly available S. aureus genomes recovered from LA and to analyze possible associations with specific S. aureus lineages, antimicrobial resistance genes (ARGs) and plasmid rep types.METHODS: A total of 1331 publicly available S. aureus genome assemblies recovered from 13 LA countries between 1978 and 2018 were queried for the presence of ARGs, rep and qac genes (qacA/B, smr-qacC, qacE, qacEdelta1, qacG, qacH, qacJ, qacL) using ABRicate v1.0.1 with resfinder, plasmidfinder and a custom qac gene database, respectively. The MLST types were obtained using PathogenWatch.RESULTS: The most prevalent S. aureus lineages among the genomes analyzed were CC5 (38.9%, 519/1331), CC30 (21.9%, 292/1331), and CC8 (15.4%, 206/1331); 58% (772/1331) of strains harbored mecA (MRSA).The qac genes were found in 6.31% (84/1331) of the genomes recovered from 9 countries (Argentina, Brazil, Chile, Colombia, Ecuador, Guatemala, Mexico, Peru, Venezuela) between 1996-2018, mainly in MRSA (61/84, 72.6%) and distributed in 8 clonal complexes: CC5 (36/84), CC30 (23/84), CC8 (12/84), CC1 (8/84), CC45(1/84), CC12(1/84), CC121(1/84), CC97(1/84), and ST7 (1/84). Most genomes carrying qac genes (72/84) presented 2 or more ARGs.Most prevalent genes were smr_qacC (48/84), present in all CC described above except CC45, mainly in strains with rep13 (34/48), rep16 (30/48), rep19 (24/48), and/or rep21 (23/48); and qacA (23/84) present in strains from CC5, CC8, CC1, and CC45, mainly with rep20 (19/23), rep16 (17/23), and/or rep19 (15/23).Less prevalent genes were qacJ (11/84) in strains from CC5, CC8, CC30 and CC1, mainly with rep21 (10/11) and rep16 (8/11); and qacG (2/84) in strains from CC5 and CC8.CONCLUSION: There is a low prevalence of qac genes in publicly available LA S. aureus genomes. Their presence in the most prevalent lineages from the region highlights the need for their surveillance, considering the actual widespread use of antiseptics and its possible role in the selection of multidrug resistant high-risk S. aureus clones.