INVESTIGADORES
MOLLERACH Marta Eugenia
congresos y reuniones científicas
Título:
Molecular Epidemiology of Community-Acquired MRSA causing Skin and Skin Structure Infections in Argentina
Autor/es:
FERNÁNDEZ S; GARDELLA N; LOPEZ FURST MJ; DE VEDIA L; STRYJEWSKI ME; GANAHA MC; PRIETO S; CARBONE E; LISTA N; ROTRYING F; MOLLERACH M
Lugar:
San Francisco
Reunión:
Conferencia; 52nd Interscience Conference on Antimicrobial Agentes and Chemotherapy; 2012
Institución organizadora:
American Society for Microbiology
Resumen:
Background Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is a leading cause of acute bacterial skin and skin structure infections (ABSSSI). In Argentina CA-MRSA ST5, SCCmec IV, PVL+, spat311, has been predominant. We conducted a prospective observational multicenter study to re-evaluate molecular characteristics of ABSSSI caused by CA-MRSA in Argentina. Methods Patients ≥14 years-old without significant healthcare contact presenting with ABSSSI and culture obtained were enrolled from March-2010 to October-2011at 19 centers. Antimicrobial susceptibility testing was performed by disk diffusion according to CLSI. Detection of mecA, Panton-Valentine (PVL) genes and SCCmec typing were done by PCR. Isolates were genotyped by spa typing, PFGE and MLST. Results Overall 311 patients with ABSSSI were enrolled. Mean age was 38.8 (±18) years; most common lesions were furuncles (36%), abscesses (34%) and cellulitis (25%). MRSA was isolated in 70% of patients (218/311). Most isolates were susceptible to erythromycin (86%), clindamycin (89%), gentamicin (92%), quinolones (95%), rifampin (99%), trimethoprim/sulfamethoxazol (99%) and all were susceptible to minocycline and linezolid. Overall 128 isolates were available for molecular analysis, PVL genes were present in 99%, and mecA was confirmed in all isolates. PFGE, ST, SCCmec type and spa type are shown in Table. Conclusions This study revealed that ST30 CA-MRSA clone has replacedST5 clone and is now the most prevalent CA-MRSA in Buenos Aires, Argentina.