INVESTIGADORES
MOLLERACH Marta Eugenia
artículos
Título:
Cefotaxime-Hydrolysing B-Lactamases in Morganella morganii
Autor/es:
POWER P; RADICE M; BARBERIS C; DE MIER C; MOLLERACH M; MALTAGLIATTI C; VAY C; FAMIGLIETTI A; GUTKIND
Revista:
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES : OFFICIAL PUBLICATION OF THE EUROPEAN SOCIETY OF CLINICAL MICROBIOLOGY.
Editorial:
Springer-Verlag
Referencias:
Año: 1999 vol. 18 p. 743 - 747
ISSN:
0934-9723
Resumen:
The frequency of enterobacterial isolates with high resistance to expanded-spectrum B-lactam antibiotics (mainly cefotaxime or ceftriaxone) has increased notoriously in Argentina, mainly because of the spread of extended-spectrum b-lactamases. The aim of this work was the study of extended-spectrum blactamases in several Morganella morganii isolates with unusually high resistance to ceftriaxone. These strains produced at least two b-lactamases, of apparent pIs of 5.4 and 8.2, molecular weight 23 000, well inhibited by clavulanate, compatible with a broad-spectrum b-lactamase perhaps TEM-1 and an extended-spectrum blactamase, respectively. The extended-spectrum blactamase was identified as a CTX-M-type b-lactamase probably CTX-M-2 by polymerase chain reaction, restriction profile analysis and DNA-DNA hybridisation. The remaining isolates studied produced either the broad-spectrum b-lactamase plus the ubiquitous AmpC B-lactamase (13 strains), or the AmpC b-lactamase only (10 strains).
