INVESTIGADORES
MOLLERACH Marta Eugenia
artículos
Título:
Characterization of the galU gene of Streptococcus pneumoniae encoding a Uridine diphosphoglucose pyrophosphorylase : a gene essential for capsular polysaccharide biosynthesis
Autor/es:
MOLLERACH M; LÓPEZ R; GARCÍA E
Revista:
JOURNAL OF EXPERIMENTAL MEDICINE
Editorial:
Rockefeller University Press
Referencias:
Año: 1998 vol. 188 p. 2047 - 2056
ISSN:
0022-1007
Resumen:
The galU gene of Streptococcus pneumoniaehas been cloned and sequenced.Escherichia coli cells harboring the recombinant plasmid pMMG2 (galU) overproduced a protein that has been shown to correspond to a uridine -triphosphate:glucose-1-phosphate uridylyltransferase (uridine diphosphoglucose [UDP-Glc] pyrophosphorylase) responsible for the synthesis of UDP-Glc, a key compound in the biosynthesis of polysaccharides. A gene very similar to the S. pneumoniae galU has been found in a partial nucleotide sequence of the Streptococcus pyogenes genome. Knockout galU mutants of type 1 pneumococci are unable to synthesize a detectable capsule. An identical result was found in type 3 S. pneumoniae cells in spite of the fact that these bacteria contain a type-specific gene (cap3C) that also encodes a UDP-Glc pyrophosphorylase. Since eukaryotic UDP-Glc pyrophosphorylases appear to be completely unrelated to their prokaryotic counterparts, we postulate that GalU may be an appropriate target for the search of new drugs to control the pathogenicity of bacteria like pneumococcus and S. pyogenes.