Detoxification and Antioxidant Responses in Diverse Organs of Jenynsia multidentata Experimentally Exposed to 1,2- and 1,4-Dichlorobenzene.

MONFERRÁN, M. V.; PESCE, S. F.; CAZENAVE, J.; WUNDERLIN, D. A.

ENVIRONMENTAL TOXICOLOGY

Wiley

Lugar: USA, NJ; Año: 2008 vol. 23 p. 184 - 192

1520-4081

ABSTRACT: We report changes in activities of detoxification and antioxidant enzymes as well as lipid peroxidation levels in liver, gills, and brain of Jenynsia multidentata exposed to 1,2- and 1,4-dichlorobenzene (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L21 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L21 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L21 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- levels in liver, gills, and brain of Jenynsia multidentata exposed to 1,2- and 1,4-dichlorobenzene (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L21 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L21 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- (DCB). Fish were captured at an unpolluted area, transported to the laboratory, and acclimated previous to experiments. Exposures were carried out using 1,2-DCB at 0.5, 1, 5, and 10 mg L21 and 1,4-DCB at 0.05, 0.1, 1, and 5 mg L21. After 24-h exposure, fish were sacrificed and dissected separating liver, gills, and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, glutathione peroxidase, catalase as well as detoxification system by measuring glutathione-S-transferase (GST) activity. Additionally, thiobarbituric acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- acid reactive substances (TBARS) method was used to evaluate the peroxidation of lipids. No changes in GSTactivity were found in liver of fish exposed to DCBs but in gills and brain of exposed fish, with an ear- and brain of each fish. Organs were used for enzyme extractions, evaluating antioxidant system through the assay of glutathione reductase, guaiacol peroxidase, gl