INVESTIGADORES
WUNDERLIN Daniel Alberto
artículos
Título:
Changes in Swimming Activity of Jenynsia multidentata Fed with Microcystin-RR. New Evidences on the Energetic Cost of Detoxification
Autor/es:
CAZENAVE, J.; NORES, M.L.; MICELI, M.; DÍAZ, M. P.; WUNDERLIN, D. A.; BISTONI, M.A.
Revista:
WATER RESEARCH
Editorial:
ELSEVIER-IWA
Referencias:
Lugar: USA; Año: 2008 vol. 42 p. 1299 - 1307
ISSN:
0043-1354
Resumen:
Abstract: We report the effects of sublethal doses of microcystin-RR (MC-RR) on swimming activity of Jenynsia multidentata as well as the simultaneous response of its detoxication system by measuring Glutathion S-transferase (GST) activities in liver and brain of fish. MC-RR was applied on the food pellets at doses of 0.01, 0.1 and 1 ìg*g-1. Swimming activity was recorded by using a computer-based image processing system, which facilitates quantification of two measures of fish swimming behaviour (average velocity, movement percentage). Results show that low levels of cyanotoxin increased the swimming activity, while the highest dose used produced significant changes with respect to control group only during the last hours of exposure, when the swimming activity was decreased. On the other hand, GST activity was significantly increased only in liver and brain of fish fed with the highest MC-RR dose. Both results suggest that fish are reacting to the stress caused by low doses of MC-RR by increasing their swimming activity, raising further questions on the probable neurotoxicity of MCs, and presenting the behavioural change as a good biomarker of early toxic stress. On the other hand, fish reduced it swimming speed at the highest MC-RR dose, when the detoxication activity began, which can be hypothesized as a reallocation of its energy, favoring detoxication over swimming activity. Swimming activity was recorded by using a computer-based image processing system, which facilitates quantification of two measures of fish swimming behaviour (average velocity, movement percentage). Results show that low levels of cyanotoxin increased the swimming activity, while the highest dose used produced significant changes with respect to control group only during the last hours of exposure, when the swimming activity was decreased. On the other hand, GST activity was significantly increased only in liver and brain of fish fed with the highest MC-RR dose. Both results suggest that fish are reacting to the stress caused by low doses of MC-RR by increasing their swimming activity, raising further questions on the probable neurotoxicity of MCs, and presenting the behavioural change as a good biomarker of early toxic stress. On the other hand, fish reduced it swimming speed at the highest MC-RR dose, when the detoxication activity began, which can be hypothesized as a reallocation of its energy, favoring detoxication over swimming activity. ìg*g-1. Swimming activity was recorded by using a computer-based image processing system, which facilitates quantification of two measures of fish swimming behaviour (average velocity, movement percentage). Results show that low levels of cyanotoxin increased the swimming activity, while the highest dose used produced significant changes with respect to control group only during the last hours of exposure, when the swimming activity was decreased. On the other hand, GST activity was significantly increased only in liver and brain of fish fed with the highest MC-RR dose. Both results suggest that fish are reacting to the stress caused by low doses of MC-RR by increasing their swimming activity, raising further questions on the probable neurotoxicity of MCs, and presenting the behavioural change as a good biomarker of early toxic stress. On the other hand, fish reduced it swimming speed at the highest MC-RR dose, when the detoxication activity began, which can be hypothesized as a reallocation of its energy, favoring detoxication over swimming activity.