INVESTIGADORES
WUNDERLIN Daniel Alberto
artículos
Título:
Effects of Microcystin-LR on the Expresion of P-glycoprotein in Jenynsia multidentata.
Autor/es:
AMÉ, M. V.; BARONI, M.V.; GALANTI, L.N.; BOCCO, J.L.; WUNDERLIN, D. A.
Revista:
CHEMOSPHERE
Editorial:
ELSEVIER
Referencias:
Lugar: AMSTERDAM; Año: 2009 vol. 74 p. 1179 - 1186
ISSN:
0045-6535
Resumen:
The multixenobiotic resistance phenomenon (MXR) related to the P-glycoprotein multidrug transporter
protein (P-gp) has been identified and characterized in several aquatic organisms. In the present work,
we prove the presence of a P-gp in liver, gills and brain of Jenynsia multidentata by Western Blot and
RTPCR. A 170 kDa protein has been found in liver and gills while in brain a 80 kDa protein has been
detected. The partial nucleotide sequence obtained in this autochthonous fish showed high similarity
ranging from 83% to 92% with other fishes. In addition, P-gp expression in this fish was evaluated after
time and dose-dependent exposures to the cyanotoxin microcystinLR. Individuals were exposed to
MCLR at concentrations of 2, 5 and 10 lg L1 for 24 h and for 6, 12 and 24 h at 2 lg L1 MCLR.
Changes in P-gp expression were observed in liver, gills and brain. However, this response was tissue
specific. Only in gills of J. multidentata P-gp expression, measured either by real-time RTPCR or Western
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Changes in P-gp expression were observed in liver, gills and brain. However, this response was tissue
specific. Only in gills of J. multidentata P-gp expression, measured either by real-time RTPCR or Western
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
RTPCR. A 170 kDa protein has been found in liver and gills while in brain a 80 kDa protein has been
detected. The partial nucleotide sequence obtained in this autochthonous fish showed high similarity
ranging from 83% to 92% with other fishes. In addition, P-gp expression in this fish was evaluated after
time and dose-dependent exposures to the cyanotoxin microcystinLR. Individuals were exposed to
MCLR at concentrations of 2, 5 and 10 lg L1 for 24 h and for 6, 12 and 24 h at 2 lg L1 MCLR.
Changes in P-gp expression were observed in liver, gills and brain. However, this response was tissue
specific. Only in gills of J. multidentata P-gp expression, measured either by real-time RTPCR or Western
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Changes in P-gp expression were observed in liver, gills and brain. However, this response was tissue
specific. Only in gills of J. multidentata P-gp expression, measured either by real-time RTPCR or Western
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Jenynsia multidentata by Western Blot and
RTPCR. A 170 kDa protein has been found in liver and gills while in brain a 80 kDa protein has been
detected. The partial nucleotide sequence obtained in this autochthonous fish showed high similarity
ranging from 83% to 92% with other fishes. In addition, P-gp expression in this fish was evaluated after
time and dose-dependent exposures to the cyanotoxin microcystinLR. Individuals were exposed to
MCLR at concentrations of 2, 5 and 10 lg L1 for 24 h and for 6, 12 and 24 h at 2 lg L1 MCLR.
Changes in P-gp expression were observed in liver, gills and brain. However, this response was tissue
specific. Only in gills of J. multidentata P-gp expression, measured either by real-time RTPCR or Western
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Changes in P-gp expression were observed in liver, gills and brain. However, this response was tissue
specific. Only in gills of J. multidentata P-gp expression, measured either by real-time RTPCR or Western
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
lg L1 for 24 h and for 6, 12 and 24 h at 2 lg L1 MCLR.
Changes in P-gp expression were observed in liver, gills and brain. However, this response was tissue
specific. Only in gills of J. multidentata P-gp expression, measured either by real-time RTPCR or Western
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
J. multidentata P-gp expression, measured either by real-time RTPCR or Western
Blot, was significantly higher compared to controls at most tested times and doses. A 3-fold
increase with respect to controls was found at 12 h by RTPCR and after 24 h by Western Blot. In
dose-dependent experiments the maximum P-gp expression was observed at 2 lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.
lg L1 MCLR, measured
by both RTPCR and Western Blot. In the liver, P-gp protein levels were significantly increased
after 24 h of exposure, at every toxin dose tested. Thus, probably longer exposures would show also
significant increases in this tissue.
Considering these results we can propose that P-gp belongs to the defence system involved in the
response to MCLR in J. multidentata.J. multidentata.