INVESTIGADORES
WUNDERLIN Daniel Alberto
artículos
Título:
Behavioral swimming effects and acetylcholinesterase activity changes in Jenynsia multidentata exposed to chlorpyrifos and cypermethr in individually and in mixtures
Autor/es:
BONANSEA R.I; WUNDERLIN D.A.; AMÉ, M.V.
Revista:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Editorial:
ELSEVIER
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 129 p. 311 - 319
ISSN:
0147-6513
Resumen:
The pesticidescypermethrin(CYP)andchlorpyrifos(CPF)werefoundtogetherinwaterbodieslocatedinagricultural andurbanareas.However,theimpacttonon-targetbiotafromexposuretomixtureshasreceivedlittleattention.Inthecurrentstudy,weevaluatedchangesinswimmingbehaviorandcholi-nesteraseenzymesactivityin Jenynsia multidentata, to investigatethepossibleeffectsofthesein-secticides individuallyandinmixtures.Moreover,differencesbetweentechnicalandcommercialmix-tures ofthepesticideswereevaluated.Femalesof J. multidentata wereexposedover96-htoCYP(0.04and 0.4 mg L1), CPF(0.4and4 mg L1), individuallyandinatechnicalandcommercialmixtures.Swimming behaviorwasrecordedafter24hand96hofexposure.Also,wemeasuredcholinesteraseenzymes activityinbrainandmuscleafter96hofexposure.ExposuretoCYPincreasedtheexploratoryactivity of J. multidentata in theupperareaoftheaquarium.FishexposedtoCPF(4 mg L1) showedadecrease inswimmingactivityandanincreaseinthetimespentatthebottomoftheaquarium.Inter-estingly, fish exposedtothetechnicalandcommercialmixtureofCYPandCPFdisplayedadifferentbehaviorbasedontheconcentrationofexposure.LowconcentrationofpesticideselicitedanincreaseinJ. multidentata swimming activitywithpreferencefortheupperareaoftheaquarium,andhighcon-centrations causeddecreaseinswimmingactivitywithpreferenceforthebottomareaoftheaquarium.Based ontheresponseofcholinesteraseenzymes,acetylcholinesteraseinmusclewasmoresensitivetoexposuretoCYP,CPFandtheirmixturesthaninbrain.Adecreaseinswimmingbehaviorcorrelatessignificantly withtheinhibitionofacetylcholinesteraseactivityinmuscleof J. multidentata exposedtohigh concentrationsofpesticides.These resultsdrawattentiontotheneedofmorestudiesonthepotentialecotoxicologicalimpactofpesticides anditsmixturesatenvironmentalrelevantconcentrations.
