Copper Induced Response of Physiological Parameters and Antioxidant Enzymes in the Aquatic Macrophyte Potamogeton pusillus.

MONFERRÁN, M. V.; SÁNCHEZ AGUDO, J.; PIGNATA, M.L.; WUNDERLIN, D.A.

ENVIRONMENTAL POLLUTION

ELSEVIER

Lugar: Amsterdam; Año: 2009 vol. 157 p. 2570 - 2576

0269-7491

Bioaccumulation and toxicity of copper was evaluated on Potamogeton pusillus L. The effect of copper (5–100 mg L1) applied for several days was assessed by measuring changes in the chlorophyll’s, phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after (5–100 mg L1) applied for several days was assessed by measuring changes in the chlorophyll’s, phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after (5–100 mg L1) applied for several days was assessed by measuring changes in the chlorophyll’s, phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after (5–100 mg L1) applied for several days was assessed by measuring changes in the chlorophyll’s, phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after (5–100 mg L1) applied for several days was assessed by measuring changes in the chlorophyll’s, phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after (5–100 mg L1) applied for several days was assessed by measuring changes in the chlorophyll’s, phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after Potamogeton pusillus L. The effect of copper (5–100 mg L1) applied for several days was assessed by measuring changes in the chlorophyll’s, phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after –100 mg L1) applied for several days was assessed by measuring changes in the chlorophyll’s, phaeophytin’s, malondialdehyde, electrical conductivity, glutathione peroxidase (GPX), glutathione reductase (GR) and guaiacol peroxidase (POD) activities. Plants accumulated copper with a maximum of 162 mg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated aftermg g1 dw after 7-days exposure at 100 mg L1, however most of the metal was accumulated after 1-day exposure. The toxic effect caused by Cu was evident by the reduction of photosynthetic pigments, increase of malondialdehyde and electrical conductivity. P. pusillus shows Cu-induced oxidative stress by modulating antioxidant enzymes like GPX, GR and POD. Antioxidant enzymes activity increased significantly after exposure to 40 mg L1 during 24 h, followed by a drop at longer times. Thus, P. pusillus is proposed as a good biomonitor for the assessment of metal pollution in aquatic ecosystems. proposed as a good biomonitor for the assessment of metal pollution in aquatic ecosystems. proposed as a good biomonitor for the assessment of metal pollution in aquatic ecosystems. proposed as a good biomonitor for the assessment of