Bioconcentration of carbamazepine and other pharmaceuticals in fish under field and controlled laboratory experiments. Evidences of carbamazepine metabolization by fish.

VALDÉS M.E; HUERTA B; WUNDERLIN, D. A.; BISTONI, M.A.; BARCELÓ D; RODRÍGUEZ-MOZAZ S

SCIENCE OF THE TOTAL ENVIRONMENT

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015

0048-9697

There is a growing interest in evaluating the presence of pharmaceuticalresidues and theirmetabolites in aquatic biota. In this study, twentypharmaceuticals, including carbamazepine (CBZ) and two metabolites, wereanalyzed in homogenates of two fish species (Gambusia affinis and Jenynsiamultidentata)captured in polluted areas of the Suquía River (Córdoba, Argentina). The twentytarget pharmaceuticalswere found in G. affinis, while only fifteen weredetected in J. multidentata. We observed a noticeable difference in theaccumulation pattern of both fish species, suggesting different pathways forthe bioaccumulation of polar pharmaceuticals in each fish. In order toinvestigate uptake and tissue distribution of pharmaceuticals, a detailed studywas performed under controlled laboratory conditions in J. multidentata,exposed to CBZ. CBZ and two of its metabolites (carbamazepine- 10,11-epoxide ? CBZ-EPand 2-hydroxycarbamazepine ? 2-OH-CBZ) were monitored in five organs of fish underlaboratory exposure. To our knowledge, this is the first report on the presenceof CBZ and its metabolite 2-OH-CBZ in gills, intestine, liver, brain and muscleof fish, while the metabolite carbamazepine-10,11-epoxide (CBZ-EP) was detectedin gills and muscle. A ratio CBZ-EP/CBZ close to 0.1 suggests that gills andmuscle of J. multidentata could metabolize CBZ through the CBZ-EP pathway.