Degradation Of Lindane By A Native Bacteria Consortium Isolated From Contaminated River Sediment.

PESCE, S.F; WUNDERLIN, D. A.

INTERNATIONAL BIODETERIORATION & BIODEGRADATION

ELSEVIER

Año: 2004 vol. 54 p. 255 - 260

0964-8305

The aerobic biodegradation of lindane (
-hexachlorocyclohexane) by a consortium of acclimated bacteria from sediment at a polluted site on the Suquia River, Cordoba, Argentina, is reported. The bacteria were acclimated for 30 days under aerobic conditions, using a minimal culture medium containing lindane (0:034 mM) as sole carbon source. Growth of the bacterial consortium decreased at a lindane concentration of 1:03 mM and was totally inhibited at 2:41 mM. The consortium showed initial lindane degradation rates of 4:92×10.3, 11:0×10.3
-hexachlorocyclohexane) by a consortium of acclimated bacteria from sediment at a polluted site on the Suquia River, Cordoba, Argentina, is reported. The bacteria were acclimated for 30 days under aerobic conditions, using a minimal culture medium containing lindane (0:034 mM) as sole carbon source. Growth of the bacterial consortium decreased at a lindane concentration of 1:03 mM and was totally inhibited at 2:41 mM. The consortium showed initial lindane degradation rates of 4:92×10.3, 11:0×10.3:034 mM) as sole carbon source. Growth of the bacterial consortium decreased at a lindane concentration of 1:03 mM and was totally inhibited at 2:41 mM. The consortium showed initial lindane degradation rates of 4:92×10.3, 11:0×10.3:03 mM and was totally inhibited at 2:41 mM. The consortium showed initial lindane degradation rates of 4:92×10.3, 11:0×10.3 and 34:8 × 10.3 mM h.1 when exposed to lindane concentrations of 0.069, 0.137 and 0:412 mM, respectively. Chloride concentration increased during aerobic biodegradation, indicating lindane mineralization. A metabolite identi;ed as
-2,3,4,5,6-pentachlorocyclohexene appeared during the ;rst 24 h of biodegradation. Four di<erent bacteria, identi;ed as Sphingobacterium spiritivorum, Ochrobactrum anthropi,:8 × 10.3 mM h.1 when exposed to lindane concentrations of 0.069, 0.137 and 0:412 mM, respectively. Chloride concentration increased during aerobic biodegradation, indicating lindane mineralization. A metabolite identi;ed as
-2,3,4,5,6-pentachlorocyclohexene appeared during the ;rst 24 h of biodegradation. Four di<erent bacteria, identi;ed as Sphingobacterium spiritivorum, Ochrobactrum anthropi,
-2,3,4,5,6-pentachlorocyclohexene appeared during the ;rst 24 h of biodegradation. Four di<erent bacteria, identi;ed as Sphingobacterium spiritivorum, Ochrobactrum anthropi,Sphingobacterium spiritivorum, Ochrobactrum anthropi, Bosea thiooxidans and Sphingomonas paucimobilis, were isolated. Pure strains of B. thiooxidans and S. paucimobilis degraded lindane after 3 days of aerobic incubation. This is the ;rst report of lindane biodegradation by B. thiooxidans.and Sphingomonas paucimobilis, were isolated. Pure strains of B. thiooxidans and S. paucimobilis degraded lindane after 3 days of aerobic incubation. This is the ;rst report of lindane biodegradation by B. thiooxidans.B. thiooxidans.