INVESTIGADORES
WUNDERLIN Daniel Alberto
artículos
Título:
Urban propolis from San Juan province (Argentina). Ethnopharmacological uses and antifungal activity against Candida and dermatophytes.
Autor/es:
AGUERO, M.B.; SVETAZ, L.; BARONI, M.V.; LIMA, B; LUNA, L.; ZACCHINO, S.; SAAVEDRA, P.; WUNDERLIN D.A.; FERESIN, G.E.; TAPIA, A.
Revista:
Industrial Crops and Products
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 57 p. 166 - 173
ISSN:
0926-6690
Resumen:
Propolis is widely used in the folk medicine of San Juan province (Argentina) to treat several diseases,including cold, cough, muscle aches and superficial mycoses. We report the in vitro antifungal activity ofurban propolis, evaluated with CLSI protocols in addition to the evaluation of their chemical profile byHPLC-ESI-MS/MS techniques.The dermatophytes Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum werethe most susceptible species and guided the fractionation of urban propolis, which was performed withSephadex LH-20 leading to eight fractions (I?VIII). These fractions showed high antifungal activitiesagainst dermatophytes (MICs = 16.0?62.5 g/mL) and yeasts (MICs = 31.2?125 g/mL) being III, V and VIthe most active ones (MIC100= 16?31.2 g/mL). They also, showed fungicidal capacity (a condition highlyappreciated in antifungal drugs to avoid recurrence) with MFC values between 31.2 and 62.5 g/mL.From the most active fractions, two lignans: 3-methyl-nordihydroguaiaretic acid (MNDGA) (1), andnordihydroguaiaretic acid (NDGA) (2), in addition to three flavonoids: chrysin (3), pinocembrin (4) andgalangin (5), were isolated and quantified by HPLC?PDA-MS/MS as the main antifungal compounds.Lignans 1 and 2 showed strong activities against T. mentagrophytes, T. rubrum and Microsporum gypseum(MICs between 31.2 and 62.5 g/mL), and 1 showed strong activity against Candida albicans, Candida trop-icalis and Cryptococcus neoformans (MICs between 31.2 and 62.5 g/mL). Regarding flavonoids, all yeastswere sensitive to 5 (MIC = 31.2 g/mL), whereas the dermatophytes T. mentagrophytes and T. rubrum andall yeasts were moderately inhibited by 4 (MIC = 31.2?250 g/mL). Finally, chrysin (3) showed low activ-ity against yeasts and dermatophytes (MIC = 250 g/mL). These results support that Argentinean urbanpropolis, which are frequently used by beekeepers for the preparation of syrups, tinctures and creams, arevaluable natural product for the improving of human health, particularly fungal infections. It is also wor-thy to take into account that its chemical composition contains mainly two antifungal lignans, associatedwith the medicinal Larrea genus.