INVESTIGADORES
WUNDERLIN Daniel Alberto
artículos
Título:
Antioxidant, phase II and III responses induced by lipoic acid in the fish Jenynsia multidentata (Anablapidae) and its influence on endolsulfan accumulation and toxicity
Autor/es:
MONSERRAT, J. M.; GARCÍA, M.L.; VENTURA-LIMA, J.; GONZALEZ, M.; BALLESTEROS, M.L.; MIGLIORANZA, K.; AMÉ, M. V.; WUNDERLIN D.A.
Revista:
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 108 p. 8 - 15
ISSN:
0048-3575
Resumen:
Antioxidants like lipoic acid (LA) are known to trigger augmented
antioxidant and phase II and III responses. This study aimed to evaluate
the effect of LA in P-glycoprotein (Pgp) expression,
glutathione-S-transferase (GST) activity, total antioxidant competence,
levels of lipid peroxides (TBARS) and accumulation of the organochlorine
insecticide endosulfan (Endo: α-, β-isomers and sulfate metabolite) in
different organs of the fish Jenynsia multidentata. One hundred and twenty females (1.55 ± 0.07 g) were fed during 8 days with (n = 60) or without (n = 60)
a LA enriched ration (6000 mg/kg). Four experimental groups were
defined: −LA/−Endo; +LA/−Endo; −LA/+Endo; and +LA/+Endo. Endo groups
were exposed during 24 h to 1.4 μg of insecticide/L. Results showed that
only LA induced a significant increment in liver Pgp expression. GST
activity was augmented in liver after exposure to LA or Endo. TBARS
levels were lowered in liver and gills after LA pre-treatment. Total
antioxidant capacity was lowered in liver of Endo exposed fish, a result
that was reversed by LA pre-treatment. It is concluded that LA induced
the expected effects in terms of Pgp expression, GST activity and
reduced TBARS levels although favored α-Endo accumulation in brain.
However, the Endo metabolism to the more persistent endosulfan sulfate
was not facilitated by LA pre-treatment.