INVESTIGADORES
WUILLOUD Rodolfo German
congresos y reuniones científicas
Título:
Solvent-free reversed-phase ion-pairing liquid chromatography coupled to atomic fluorescence spectrometry for speciation analysis of arsenic in complex matrices
Autor/es:
MONASTERIO, ROMINA; LONDONIO, AGUSTIN; FARIAS, SILVIA; SMICHOWSKI, PATRICIA; WUILLOUD, RODOLFO G.
Lugar:
Mar del Plata
Reunión:
Simposio; ELEVENTH RIO SYMPOSIUM ON ATOMIC SPECTROMETRY; 2010
Institución organizadora:
Universidad de Buenos Aires y Comisión Nacional de Energía Atómica
Resumen:
A number of phenylarsonic compounds have been shown to control cecal coccidiosis in poultry, and also act as growth promoters, providing improved feed conversion, better feathering and increased egg production and pigmentation.1,2 Roxarsone (3-nitro-4-hydroxyphenylarsonic acid; ROX), nitarsone (4-nitrophenylarsonic acid; NIT) and p-arsanilic acid (4-aminophenylarsonic acid; p-ASA) are the most commonly used aromatic organoarsenicals as feed additives. Arsenic-contaminated poultry litter could be applied as fertilizer in crop fields leading to elevated arsenic concentrations in soils and leaching water.3 A novel method has been developed to detect organic arsenic animal feed additives including ROX, p-ASA and NIT, as well as other arsenic species such as DMA and MMA using ion-pairing high-performance liquid chromatography coupled to atomic fluorescence spectrometry (IP-HPLC-AFS). An isocratic reversed-phase (RP) HPLC method with a mobile phase containing citric acid and sodium hexanesulfonate was developed using a C18 column. The use of a solvent-free mobile phase turns this methodology into a low cost and environmentally friendly alternative. The effect of type and concentration of ion-pairing reagents (sodium hexanesulfonate, tetrabutylammonium bisulfate and perfluoroheptanoic acid), and pH of mobile phase on chromatographic resolution and repeatability of retention time of the different arsenic species was examined. The method was applied for arsenic speciation studies in arugula animal feed, urine and chicken liver samples. 1. S. A. Pergantis, E. M. Heithmar and T. A. Hinners, Analyst, 1997, 122, 1063-1068. 2. K. Baba, T. Arao, Y. Maejima, E. Watanabe, H. Eun and M. Ishizaka, Anal. Chem., 2008, 80, 5768-5775. 3. D. Sánchez-Rodas, J. Luis Gómez-Ariza and V. Oliveira, Anal. Bioanal. Chem., 2006, 385, 1172-1177.